Batı Karadeniz Tıp Dergisi

Öz

Aim: Bisphenols (BPs) are potential hormone disruptors which the general population is exposed in many commonly used products.
Bisphenol A (BPA) is an industrial chemical that has been used for many years in the production of some plastics and resins, despite its
adverse effects on the body's metabolic systems. One of the most important organs affected by toxic agents such as BPA and also from
environmental pollutants is the kidneys. Ellagic acid (EA) is a phytochemical polyphenol compound, and many studies report that EA exhibits
antioxidant, anti-mutagenic, anti-cancer and anti-apoptotic properties. The aim of this study is to reveal the effect of EA on BPA-induced
kidney damage.
Material and Methods: In this study, 24 male rats were divided into four groups as Control, BPA (25mg/kg for 30 days), EA (10/mg/kg
for 30 days) and BPA+EA (25mg-10mg/kg for 30 days, respectively) (n=6). At the end of the study, the weights of kidneys taken from
decapitated rats and blood urea nitrogen (BUN) and creatinine values were measured from the serum. In addition, Picro Sirius Red staining
was performed to reveal the general histological appearance of the kidneys, and immunohistochemical staining was performed using
8-hydroxydeoxyguanosine antibody to reveal the DNA damage occurring in the tissues.
Results: It was observed that the absolute and relative weights of the left kidney increased in the groups where BPA was induced, and
decreased in the BPA+EA group, similar to the control group. It was determined that blood urea nitrogen (BUN) and creatinine values
increased significantly in the BPA group compared to the control group, while the values decreased in the BPA+EA group. As a result of
staining with 8-hydroxydeoxyguanosine antibody in the glomerulus and tubule structures of the kidney tissues taken from the BPA-induced
group, intense positivity, degeneration and vacuolization in the glomerulus structure, some regional necrosis areas and deterioration of the
tubule structures were observed, while very few stained areas were observed in the EA and control groups, similar to each other. In the
group where BPA and EA were used together, protection of the tissues was observed in both the glomerulus structure and tubule structures
compared to the group where BPA was used alone, and less retention of 8-hydroxydeoxyguanosine was observed.
Conclusion: It has been concluded that the possible damage to kidney tissue caused by BPA, which individuals from all segments of society
may be exposed to, can be reduced by using EA supplements.